Extended Data Fig. 12. Hypoxia tolerance of cartilage with hemoglobin depletion.
a, TUNEL assay of cartilages of E18.5 mice cultured in vitro under hypoxia for 3 days. Quantification of all biological replicates is provided on the right. Scale bars: 50 μm. The data are mean with SEM of triplicate experiments. b, c, The humeral cartilage growth plates of E14.5 mice of the indicated genotypes were cultured in hypoxic environment (1% oxygen concentration) for 6 days, significantly more chondrocytes death occurred in the growth plates of Hba (b) or Hbb (c) homozygous knockout cartilages. The dead cell areas were marked with solid black lines. Scale bar: 50 μm. n = 3 biologically independent experiments. Error bars represent SEM. P values were calculated using one-way ANOVA tests. The exact P-values of comparison are presented in the figures, respectively.