Fig. 7.

Pals1-binding deficient PATJ mimics polarity but not cilia defects. A Western blot of wild type MDCK, MDCK∆PATJ and MDCK∆PATJ transduced with wild type mPATJ (wild-type rescue) or mPATJ∆L27. B, C Immunostainings of the indicated cell lines with ZO-1 and Pals1 reveal TJ defects and a mislocalization of Pals1 from the TJ in case of PATJ-deficient and PATJ∆L27 expressing cells, whereas wild-type PATJ rescues TJ defects. D–F Indicated cell lines were cultured in Matrigel and stained with the indicated antibodies. G Quantification of lumen formation in cysts of the indicated cell lines. Error bars are standard error of the means. H, I Immunostaining of PATJ-GFP variants demonstrates a failure of PATJ∆L27 to localize to primary cilia. J Quantification of primary cilia formation upon serum starvation. Error bars are standard error of the means. K–N Inhibition of HDAC7 by TMP195 rescues single lumen formation defects in Pals1 deficient MDCK cells but not junctional PATJ localization. Error bars are standard errors of the means. Significance was determined by one-way ANOVA test and Bonferroni correction: *p < 0.05, ns not significant. Scales bars are 20 µm in B–D and H, I, 30 µm in D–F and 50 µm in K–M