Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 25;14:6559. doi: 10.1038/s41467-023-42276-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Schematic diagram of the experimental design. b Immunohistochemical analysis of Iba1 (green) in the mPFC at P51. (Left) Representative images of the mPFC, representative tracing images (red), and images of cellular processes (green) and cell bodies (yellow) of Iba1⁺ cells. Scale bar, 50 μm (left) and 10 μm (middle and right). c The number of Iba1⁺P2ry12⁺ cells (left) and Iba1⁺P2ry12⁻ cells (middle) in the mPFC, presented as % of control. (Right) The percentage of Iba1⁺P2ry12⁻ cells among all Iba1⁺ cells in the mPFC. (n = 6 slices in 3 mice per condition). d Quantification of the ratio of cellular process area (left) and cell body size to total cell size (right) of Iba1⁺ cells. (n = 50 cells in 5 mice per condition). e Representative images of immunohistochemistry of Iba1 (green) and Casp3-p17 (red) (top left) as well as TUNEL signals (red) and DAPI (blue) (bottom left) in the mPFC at P51. Scale bar, 50 μm. f Quantification of signal intensity of Casp3-p17 in Iba1⁺ cells (left) and TUNEL (right). n = 6 slices in 3 mice per condition. c, d, f ***p < 0.001, **p < 0.01, *p < 0.05 (p values are (c) (Left) 16p11^wt;Cnr1^+/+ versus 16p11^wt;Cnr1^flox/flox: p = 0.0281, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^+/+: p = 0.0001, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p = 0.0221, 16p11^dup; Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, (Middle) 16p11^wt;Cnr1^+/+ versus 16p11^wt;Cnr1^flox/flox: p = 0.0285, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^+/+: p < 0.0001, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p = 0.0337, 16p11^dup; Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, (Right) all: p < 0.0001, (d) (Left) 16p11^wt;Cnr1^+/+ versus 16p11^wt;Cnr1^flox/flox: p < 0.0001, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^+/+: p = 0.0017, 16p11^wt; Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, 16p11^dup;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, (Right) 16p11^wt;Cnr1^+/+ versus 16p11^wt;Cnr1^flox/flox: p = 0.0225, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^+/+: p = 0.0183, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p = 0.0330, 16p11^dup;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, (f) (Left) 16p11^wt;Cnr1^+/+ versus 16p11^wt;Cnr1^flox/flox: p = 0.0016, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^+/+: p < 0.0001, 16p11^wt;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p = 0.0032, 16p11^dup;Cnr1^+/+ versus 16p11^dup;Cnr1^flox/flox: p < 0.0001, (Right) all: p < 0.0001), determined by two-way ANOVA with post hoc Tukey test. Each symbol represents one slice (a, f) and one animal (d). Data are presented as the mean ± s.e.m.