Fig. 2. Impact of infection progress on individual small molecules.
a UpSet plot demonstrating limited overlap between infection-impacted small molecules at each heart section, for each timepoint post-infection. Bars are colored by superclass from ClassyFire annotation, as implemented in MolNetEnhancer130,131. Dark circles represent intersections between groups, with the size of that intersection on top of the colored bar graph. Total number of features impacted by infection at each sampling site is represented on the left barplot. b UpSet plot demonstrating limited overlap between infection-impacted small molecules at each timepoint, for each of the heart sections. c Representative small molecules locally impacted by infection over time. Red line, p-value < 0.05 by Mann-Whitney U test, two-sided, FDR-corrected. Black line, p-value > 0.05 by Mann-Whitney U test, two-sided, FDR-corrected. Numbers on top of the lines are the corrected p-values. Boxplots represent median, upper and lower quartiles, with whiskers extending to show the rest of the distribution, except for points that are determined to be outliers by being beyond the interquartile range ±1.5 times the interquartile range. RA right atrium, LA left atrium, RVT right ventricle top, RVB right ventricle bottom, LVT left ventricle top, LVB left ventricle bottom, DPI days post-infection. N = 15 mice per group and per position. Source data are provided as a Source Data file.