Fig. 5. BNZ + Tc24 vaccine combination treatment restored more infection-perturbed small molecules than BNZ-only treatment.

All small molecules in panel (a) came from random forest analysis between naïve and infected mice at 142 DPI. Bars are colored by superclass annotation generated in Classyfire¹³⁰, implemented in MolNetEnhancer¹³¹. a Small molecules impacted by infection. b Small molecules restored by any treatment (BNZ-only or combo treatment (BNZ+Tc24-C4/E6020-SE)). c Small molecules not restored by any treatment. d Small molecules only restored by BNZ + Tc24 vaccine treatment. e Small molecules only recovered by BNZ treatment. f Small molecules commonly restored by both treatments. RA right atrium, LA left atrium, LVT left ventricle top, LVB left ventricle bottom, RVT right ventricle top, RVB right ventricle bottom. g Representative small molecules restored by different treatments. Red line, p-value < 0.05 by Mann-Whitney U test, two-sided, FDR corrected. Black line, p-value > 0.05 by Mann–Whitney U test, two-sided, FDR-corrected. Numbers on top of the lines are the corrected p-values. Boxplots represent median, upper and lower quartiles, with whiskers extending to show the rest of the distribution, except for points that are determined to be outliers by being beyond the interquartile range ±1.5 times the interquartile range. N = 15 mice per group and per position throughout the figure. Source data are provided as a Source Data file.