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. 2023 Oct 25;13:18253. doi: 10.1038/s41598-023-45255-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visfatin gene (NAMPT) and protein expression in the posterior pituitary lobe (NP) of pigs. Relative expression of visfatin mRNA and protein determined using quantitative real-time PCR and Western blot procedures, respectively, in NP tissues collected on days 2 to 3, 10 to 12, 14 to 16, 17 to 19 of the oestrous cycle (A,D), days 10 to 11, 12 to 13, 15 to 16, 27 to 28 of pregnancy (B,E) and days of early pregnancy compared to days 10 to 12 of the oestrous cycle (C,F). Right side of the figure: upper panels contain representative immunoblots (all bands shown in the figure come from the same blot; for an additional comparison at point F, where the concentrations of proteins during chosen periods of the cycle/pregnancy were compared, the artificially arranged bands were separated by a vertical line; uncropped images of visfatin and actin immunoblots are attached in the Supplementary File 1), lower panels demonstrate densitometric analysis of expression of visfatin protein relative to actin protein; results are means ± S.E.M (n = 5). Values associated with bars with different superscripts are different (one way ANOVA followed by Tukey post hoc test), capital letters indicate p < 0.05.