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. 2023 Oct 25;13:18253. doi: 10.1038/s41598-023-45255-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of GnRH (100 ng/mL), FSH (100 ng/mL), LH (100 ng/mL) and INS (10 ng/mL) on visfatin protein expression (A–D) and visfatin secretion by the porcine pituitary cells (E–H) during the oestrous cycle. The visfatin protein expression was analysed by Western blot. Results are shown as representative immunoblots (each of the panels represents one blot; uncropped images of visfatin and actin immunoblots are attached in the Supplementary File 1) and bar graphs with densitometry measurement of relative visfatin protein content normalised with actin protein. Visfatin concentration in culture media was evaluated based on ELISA assay. Results are means ± S.E.M (n = 5). Values associated with bars with different superscripts are different; capital letters indicate p < 0.05. C control, GnRH gonadotrophin-releasing hormone, FSH follicle-stimulating hormone, LH luteinising hormone, INS insulin.