. 2023 Oct 25;13:18253. doi: 10.1038/s41598-023-45255-4

Table 1.

Primers specification, reaction mixture composition and reaction conditions used in the study for the quantitative real-time PCR.

Gene symbol	Primer sequence	Accession number	Reaction mixture composition	Reaction conditions	Reference
NAMPT	F: 5′-CCAGTTGCTGATCCCAACAAA-3′ R: 5′-AAATTCCCTCCTGGTGTCCTATG-3′	XM_003132281.5	Power SYBR Green—12.5 µL cDNA—20 ng Forward primer—300 nM Reverse primer—300 nM H₂O—up to a total volume of 20 μL	Activation and initial denaturation: 95 °C, 10 min 40 cycles of: denaturation: 95 °C, 15 s annealing: 60 °C, 1 min	⁸⁰
UBC	F: 5′-GGAGGAATCTACTGGGGCGG-3′ R: 5′-CAGAAGAAACGCAGGCAAACT-3′	XM_003483411.3	Power SYBR Green—12.5 µL cDNA—20 ng Forward primer—400 nM Reverse primer—400 nM H₂O—up to a total volume of 20 μL	Activation and initial denaturation: 95 °C, 10 min 40 cycles of: denaturation: 95 °C, 15 s annealing: 60 °C, 1 min elongation: 72 °C, 1 min	⁸¹
18sRNA	F: 5′-TCCAATGGATCCTCGCGGAA-3′ R: 5′-GGCTACCACATCCAAGGAAG-3′	AY265350.1

Gene symbol

Primer sequence

Accession number

Reaction mixture composition

Reaction conditions

Reference

NAMPT

F: 5′-CCAGTTGCTGATCCCAACAAA-3′

R: 5′-AAATTCCCTCCTGGTGTCCTATG-3′

Power SYBR Green—12.5 µL

cDNA—20 ng

Forward primer—300 nM

Reverse primer—300 nM

H₂O—up to a total volume of 20 μL

Activation and initial denaturation: 95 °C, 10 min

40 cycles of: denaturation: 95 °C, 15 s

annealing: 60 °C, 1 min

⁸⁰

UBC

F: 5′-GGAGGAATCTACTGGGGCGG-3′

R: 5′-CAGAAGAAACGCAGGCAAACT-3′

Power SYBR Green—12.5 µL

cDNA—20 ng

Forward primer—400 nM

Reverse primer—400 nM

H₂O—up to a total volume of 20 μL

Activation and initial denaturation: 95 °C, 10 min

40 cycles of: denaturation: 95 °C, 15 s

annealing: 60 °C, 1 min

elongation: 72 °C, 1 min

⁸¹

18sRNA

F: 5′-TCCAATGGATCCTCGCGGAA-3′

R: 5′-GGCTACCACATCCAAGGAAG-3′

NAMPT visfatin, UBC ubiquitin C, 18sRNA 18 s ribosomal RNA, F forward primer, R reverse primer.