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. 2023 Oct 25;14:6779. doi: 10.1038/s41467-023-42502-0

Fig. 1. Therapeutic effect of organoid transplantation in intestinal ischemia-reperfusion (I/R).

Fig. 1

A Schematic illustration of the induction protocols for intestinal I/R and organoids transplantation. B Kaplan-Meier survival curve of organoids-transplanted and control mice after induction of intestinal I/R (n = 17 mice/group). Represent significant *p  =  0.0277 using two-tailed log-rank test. C Representative images of organoids engraftments in the small intestine 36 h after I/R injury. D Statistical analysis of intestinal crypt depth. Five different fields from each mouse were obtained for each group (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups). Represent significant *p  =  0.0198 using two-tailed student’s t test. E Quantification of pathology score at indicated time points (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups). Represent significant **p  =  0.0023 using two-tailed student’s t test. Protein concentration (F) and mRNA level (G) of IL-10 (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups). Represent significant *p  =  0.0109 using two-tailed student’s t test in (F); represent significant *p  =  0.0199 using two-tailed student’s t test in (G). H Enzyme-linked immunosorbent assay (ELISA) analysis of IL-6, IL-1β and TNF-α protein concentration in serum (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups) Represent significant p value using two-tailed student’s t test, IL-6: 0.0016; IL-1β: 0.039. I Immunofluorescence image of intestinal crypts following Ki-67 and lysozyme staining. Quantification of Ki-67+ proliferation cells (J) and lyz1+ Paneth cells (K) (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups). Ki-67+ proliferation cells: represent significant *p value = 0.0159 using two-tailed Mann–Whitney test; represent significant **p value = 0.0019 using two-tailed student’s t test. Lyz1+ Paneth cells: represent significant *p value = 0.0181 using two-tailed student’s t test. Twenty-five different crypts from each mouse were obtained for each group. Quantitative real-time PCR (qRT-PCR) analysis of intestinal tissue for Ki-67 (L) and lysozyme (M) mRNA expression (n = 4 mice for sham group with organoids transplanted, control group at 6 h and transplanted group at 36 h, n = 5 mice for the rest groups). Represent significant p value using two-tailed student’s from left to right, Ki-67: 0.0249, 0.0365; lysozyme: 0.0103, 0.0019. The statistical tests employed included: two-tailed log-rank test, two-tailed student’s t test and Mann–Whitney tests. *P < 0.05, **P < 0.01. Scale bar, 100 μm in (C) and 50 μm in (I). Each dot represents data from a single mouse ([D], [G] and [J–M]). Bar graphs represent mean ± standard deviation (SD). Source data are provided as a Source Data file.