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. 2023 Oct 25;8:161. doi: 10.1038/s41541-023-00755-2

Fig. 2. Equilibrium thermal unfolding, transient thermal stability and limited trypsin proteolytic profiles of designed immunogens.

Fig. 2

Thermal unfolding profiles. Apparent melting temperature of (a) RS2, (b) S2R, (c) S2 and (d) Spike were measured using Nano-DSF. e–h Transient thermal stability profiles. Protein samples were subjected to different temperatures (4, 37, and 50 °C) for one hour. Thermal stability of (e) RS2, (f) S2R, (g) S2 and (h) Spike was monitored using Nano-DSF. Normalized first derivative of fluorescence at 350 nm is plotted as function of temperature. i–l Proteolytic stability profile. Coomassie stained SDS-PAGE profiles of purified (i) RS2, (j) S2R, (k) S2 and (l) Spike subjected to TPCK-Trypsin proteolysis at 37 °C and 4 °C.