Fig. 5. Stability of RS2.

Characterization of lyophilized and resolubilized RS2 after incubation at 37 °C for 1 month. a–h Physiochemical characterization of RS2 in 1X PBS with equal amount (v/v) of SWE adjuvant incubated at 5 and 40 °C in polypropylene (PP) and glass vials (GV) for one month. Adjuvant properties were measured on day 0, day 7, day 14 and day 30. a Particle size, b Polydispersity Index, c Zeta Potential, d pH, e Osmolality, f Squalene content. g, h Antigenic integrity of RS2 in PBS and SWE adjuvant was measured based on binding to CR3022 using ELISA. i Day 7, j Day 14, k Day 30. Freshly thawed RS2 sample without any external modification ‘RS2 ctrl extemp’ was used as a control for undegraded antigen.