Figure 1.

Mechanism of BAM15-induced uncoupling of oxidative phosphorylation. The link between pyruvate production, the Krebs cycle, proton release, and ATP synthesis is detailed, elucidating the BAM15-induced uncoupling process. Oxidative phosphorylation involves electron transfer through the chain, creating a proton gradient for ATP synthesis. BAM15, a synthetic mitochondrial uncoupler, disrupts this coupling. By targeting the inner mitochondrial membrane, it enhances proton permeability, dissipating the gradient and uncoupling electron transport from ATP synthesis. This boosts mitochondrial respiration and energy expenditure.