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. Author manuscript; available in PMC: 2023 Oct 26.
Published in final edited form as: Cell Rep. 2023 Sep 6;42(9):113049. doi: 10.1016/j.celrep.2023.113049

Figure 7. Erosion of fast motor neuron transcriptional profiles in prdm16 mutants.

Figure 7.

(A) tSNE representing all MNs from WT (8,145), mecom mutant (4,210), and prdm16 mutant (6,493) larvae at 5 dpf. Cluster 14 was determined to be fast MNs (WT, n = 88; mecom mutant, n = 40; and prdm16 mutant, n = 65).

(B) Violin plots representing log fold expression of fast MN markers (pcp4a, calca, calb2b, scn4ba, nefma and slow MN marker (uts2d) in cluster 14 of (A) identified as normally Prdm16+ fast MNs. uts2d showed a 3 log fold change increase in prdm16 mutants (adjusted p = 0.0194).

(C) FISH of uts2d in Tg(prdm16::GFP) control and prdm16 mutant larvae at 5 dpf. Scale bars, 10 μm.

(D) Left: quantification of uts2d+ cells and GFP+ uts2d+ cells in Tg(prdm16::GFP) larvae at 5 dpf as in (C). Right: percentage of total GFP+ cells that express uts2d. Line indicates average with SEM. Unpaired t tests.

(E) FISH of satb2 in Tg(prdm16::GFP) control and prdm16 mutant larvae at 5 dpf. Scale bars, 10 μm.

(F) Left: quantification of satb2+ cells and GFP+; satb2+ cells in Tg(prdm16::GFP) larvae at 5 dpf as in (E). Right: percentage of total GFP+ cells that express satb2. Line indicates average with SEM. Unpaired t tests.