Figure 2.

Human mesenchymal stem cells (hMSCs) cultured in basal (B) or licensed (L) conditions were seeded on mineralized collagen scaffolds containing chondroitin-6-sulfate (CS6) or heparin (Hep) glycosaminoglycans with isotropic pores for 21 days in basal media. (A) Metabolic activity measured by nondestructive alamarBlue assay for each group over the 21-day culture period expressed as a fold change to a day 0 control for each treatment (basal or licensed), respectively (n = 4). Cumulative release of (B) osteoprotegerin - OPG, (C) prostaglandin E₂ - PGE2, and (D) interleukin 6 - IL-6 from hMSCs cultured in basal or licensed conditions seeded on mineralized collagen scaffolds over the 21-day period (n = 5). * denotes significance (p < 0.05) between indicated groups of same treatment (basal or licensed),** denotes significance (p < 0.05) between all groups of same treatment (basal or licensed),_^ denotes significance (p < 0.05) between indicated groups of different treatment,_^̂ denotes significance (p < 0.05) between indicated group and all other groups of other treatment, and # denotes significance (p < 0.05) of indicated group and all other groups at that time point.