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. 2023 Oct 25;24:255. doi: 10.1186/s12931-023-02550-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — TAK-242 downregulates GO-203-caused TLR4/MyD88/NF-κB pathway activation and NLRP3 inflammasome-mediated pyroptosis in OVA/LPS-induced asthmatic mouse. (A) The protein expression levels of MUC1, TLR4, MyD88, p-p65, and p65 in lung tissue were determined via immunoblotting. (B) The expression of MUC1, TLR4, and p-p65 in the bronchi were determined via immunofluorescence staining. Images were taken using a fluorescent microscope at 400× magnification. Red: MUC1; green: TLR4 and p-p65; blue: DAPI; scale bar: 50 μm. (C) The p65 protein levels in the nuclear and cytoplasmic fractions in lung tissue were analyzed via immunoblotting. (D) The protein expression levels of NLRP3, caspase-1, cleaved caspase-1, GSDMD, GSDMD-N, IL-18, and IL-1β in lung tissue were determined via immunoblotting. (E) LDH release into BALF was detected via LDH release assay. (F) The expression of NLRP3 and caspase-1 in the bronchi were determined via immunofluorescence staining. Images were taken using a fluorescent microscope at 400× magnification. Red: NLRP3; green: caspase-1; blue: DAPI; scale bar: 50 μm. LDH = lactate dehydrogenase. Data are expressed as mean ± SD (n = 6 animals per group) and were analyzed using one-way analysis of variance, *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001