Fig. 11.

SIRT1 knockout abolished the protective effects of Metrnβ overexpression in vivo. WT or SIRT-1-knockout mice were treated with Ad-Metrnβ and/or LPS. A Protein levels of SIRT1, Ferroportin, and Ferritin in mice in each group (n = 4). B H&E staining of lung tissue in each group (n = 6). C Lung wet and dry weight ratios (W/D ratios) of mice in each group (n = 6). D Lung injury scores of mice in each group (n = 6). E Levels of proinflammatory markers in lung tissue in each group (n = 6). F MDA levels in lung tissue in each group (n = 6). G GSH levels in lung tissue in each group (n = 6). H Fe²⁺ levels in mouse lung tissue in each group (n = 6). *P < 0.05 vs. the CON/WT group. #P < 0.05 vs. the LPS/WT group. NS no significant