Fig. 9.

Ferroptosis inhibition or activation abolished Metrnβ-mediated effects in vitro. A–D MLE-12 cells were transfected with Ad-Metrnβ and/or cotransfected with Gpx4 siRNA A Protein levels of Gpx4 after the cells were transfected with Gpx4 siRNA (n = 4). B Levels of proinflammatory markers in MLECs (n = 6). C The viability of MLECs (n = 6). D Fe²⁺ levels in MLECs (n = 6); MDA levels in MLECs (n = 6); GSH levels in MLECs (n = 6). *P < 0.05 vs. the CON group; #P < 0.05 vs. the LPS group. ^&P < 0.05. NS: no significant. E–G: MLE-12 cells were transfected with Metrnβ siRNA and/or treated with Fer-1. E Levels of proinflammatory markers in MLECs (n = 6). F The viability of MLECs (n = 6). G Fe²⁺ levels, MDA levels, GSH levels in MLECs (n = 6). *P < 0.05 vs. the CON group; #P < 0.05 vs. the LPS group. ^&P < 0.05. NS no significant