Fig 4.

Glutathione content, HOSCN reductase (Har) and glutathione reductase (GR) activity, and NADPH levels in WT and mutant strains. (A) Total glutathione (B), Har activity (C), GR activity, and (D) NADPH/NADP⁺ ratios were measured in bacterial lysates as described in the Materials and Methods. Data are presented as the mean ± SD from at least three independent experiments. A significant difference when compared with lysates from WT was determined by one-way ANOVA with Dunnett’s multiple comparisons test and is indicated by * for P < 0.05.