Figure 1.

The effect of crb2a ^−/− on retinal neurogenesis and lamination. Characterisation of cell types present in zebrafish retina at 56 hpf (A–H, wild type zebrafish, A′‐H′ crb2a ^m289 zebrafish). Early retinal progenitor cells were identified through staining with pax6 in WT (A and B) and crb2a ^−/− (A′ and B′) and anti‐vsx2 (C, D, C′ and D′) at 56 hpf. Anti‐ZPR1 antibody (zpr1) was used to identify the presence of cone cells (E and F) in WT; however these were absent from the mutant retina (E′ and F′) at 80 hpf. The presence of Müller cells was visualised using an anti‐glutamate synthetase antibody (GS) in WT (G and H) and mutant (G′ and H′) retina. M‐phase nuclei, visualised using an anti‐phospho‐Histone 3 antibody (PH3), were observed in both WT (I) and crb2a ^−/− (J) at 56‐hpf. All nuclei are stained with DAPI. S‐phase nuclei as visualised by 5‐ethynyl‐2’‐deoxyuridine (EdU) incorporation were observed in WT (I′) and crb2a ^−/− (J′) retina. Panels I″ and J″ are merged images of previous panels. (K) Quantification of PH3‐positive cells; *** p < 0.001. Scale bar, 50 μm.