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[Preprint]. 2023 Oct 6:rs.3.rs-3393042. [Version 1] doi: 10.21203/rs.3.rs-3393042/v1

Table 1.

Antioxidant response element (ARE) gene expression induction and mitochondrial respiratory activity as measured by oxygen consumption rate (OCR) were compared in Study 1 between CAW-treated and placebo-treated mice with experimental autoimmune encephalomyelitis by t-tests. For Study 2, Dunnett’s pairwise comparisons for ARE gene expression and Tukey’s post hoc for OCR are presented below. ANOVA results are presented in the text. Sample sizes were n = 9 per group for Study 1 and n = 10 per group for Study 2 with exceptions individually noted.

Study 1 Study 2
Outcome Placebo CAW Placebo CAW Control
ARE: NRF2 mean fold induction, SEM 1, 0.0.24 1.66, 0.31

p = 0.15
2.12, 0.43
vs control, p = 0.34
3.99, 0.90
vs placebo, p = 0.07
1, 0.23
vs CAW, p = 0.002
ARE: HMOX1 Mean fold induction, SEM 1, 0.30 1.42, 0.39

p = 0.43
1.63, 0.24
vs control, p = 0.42
2.90, 0.58
vs placebo, p = 0.047
1, 0.14
vs CAW, p = 0.002
ARE: NQO1 Mean fold induction, SEM 1, 0.29 1.31, 0.26

p = 0.46
2.03, 0.35
vs control, p = 0.27
4.09, 0.71
vs placebo, p = 0.013
1, 0.29
vs CAW, p = 0.0003
ARE: GCLC Mean fold induction, SEM 1, 0.24 1.95, 0.48

p = 0.13
1.88, 0.26
vs control, p = 0.12
3.20, 0.46
vs placebo, p = 0.016
1, 0.17
vs CAW, p = 0.0001
OCR -basal (pmol O2/min) mean SEM 133.1, 4.4

n = 3
156.8, 6.4

n = 4, p = 0.04
90.08, 3.55
vs control, p = 0.03
105.56, 4.98
vs placebo, p = 0.18
105.20, 2.99
vs CAW, p = 0.99
OCR -maximal (pmol O2/min) mean SEM 156.4, 9.2

n = 3
190.28, 5.7

n = 4, p = 0.03
112.60, 3.10
vs control, p = 0.001
140.82, 6.33
vs placebo, p = 0.0005
138.90, 5.16
vs CAW, p = 0.96
OCR- spare capacity (pmol O2/min), mean SEM 47.8, 5.0

n = 3
64.8, 2.8

n = 4, p = 0.03
22.52, 4.87
vs control, p = 0.38
35.26, 5.29
vs placebo, p = 0.28
33.62, 6.97
vs CAW, p = 0.98

GCLC, glutamate-cysteine ligase, catalytic subunit; HMOX1, heme oxygenase 1; NQO1, NAD(P)H dehydrogenase-quinone oxidoreductase 1; NRF2, nuclear factor (erythroid-derived 2)-like; SEM, standard error of the mean