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[Preprint]. 2023 Oct 4:rs.3.rs-3270539. [Version 1] doi: 10.21203/rs.3.rs-3270539/v1

Figure 1. REST is downregulated in NEPC.

Figure 1

(A) qPCR and Western blotting for indicated genes and proteins in a NEPC cell line 144-13, ADPC line LNCaP, and LNCaP-derived cell lines C4-2, NE1.3 and LNCaP-AI. Y-axis shows relative fold changes in mRNA expression, normalized to GAPDH. Error bars in PCR results represent standard deviation (s.d) of duplicate experiments. Beta actin was examined as the loading control in Western blotting. (B) REST mRNA is significantly lower in NEPC (CRPC-NE) than in adenocarcinoma CRPC (CRPC-adeno) (Beltran_NM2016)39. (C) REST mRNA in the top 25% NE-higher samples versus the bottom 25% NE-low samples of CRPC in SU2C-PCF dataset53. (D) Expression of NE marker SYP negatively correlates with that of REST in SU2C-PCF dataset. REST correlations for additional NE markers (ENO2, CHGA, CHGB, and TUBB3) are presented in Supplementary Fig. 1.