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[Preprint]. 2023 Oct 4:rs.3.rs-3270539. [Version 1] doi: 10.21203/rs.3.rs-3270539/v1

Figure 3. ADT downregulates REST in vitro and in vivo.

Figure 3

(A-B) Western blotting of REST in LNCaP and C4-2 cells treated with ADT drug MDV3100 (10uM, 72 hours), androgen DHT (25nM, 24 hours) or both. (C) Western blotting of whole cell lysates from C4-2 cells growing in regular FBS or CSS (charcoal stripped serum) for 7 days. Culturing in CSS media that is deprived of hormones is another common ADT approach in vitro. (D)Morphology of C4-2 cells growing in regular FBS or CSS. (E) RT-PCR and DNA gel electrophoresis of REST and loading control RPS18 in LNCaP xenograft tumors growing in uncastrated or castrated NOD/SCID male mice.