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[Preprint]. 2023 Oct 20:rs.3.rs-3446917. [Version 1] doi: 10.21203/rs.3.rs-3446917/v1

Table 3.

Mismatched TRB chains from multi-specific TCRs with perfectly matched TRA chains. A

Clone ID Specificity Epitope TRBV-gene Junction TRBJ-gene
Clone_81 Islet GAD65 377-396 TRBV12-4 CASSPQGGNTEAFF TRBJ1-1
Clone_566 Islet GAD65 377-396 TRBV12-4 CASSVQGGNTEAFF TRBJ1-1
P196-1 Influenza MP54 97-116 TRBV12-4 CASSLQGGNTEAFF TRBJ1-1
A

Shown are mismatched TRB chain amino acid sequence comparisons of multi-specific TCRs that recognized both GAD65 and MP54 peptides. All TCRs had identical TRA chains (TRAV29DV5-CAASRYSGGGADGLTF-TRAJ45). Sequences of GAD65 377-396 54 (HKWKLSGVERANSVTWNPHK, where bold font denotes core sequence complexed with the TCR) and MP74 97-116 55 (VKLYRKLKREITFHGAKEIS) peptides showed minimal sequence similarity by multiple sequence alignment.

B

Bold font indicates mismatches between the three TRB chains.