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[Preprint]. 2023 Oct 19:rs.3.rs-3417076. [Version 1] doi: 10.21203/rs.3.rs-3417076/v1

Figure 5. The pharmacological chaperone 2a increases SN retromer cargo protein levels in the SN of AAV-A53T injected mice:

Figure 5

A-L) Double immunofluorescence of CI-MPR (green) in TH-positive neurons (red) in the SNpc in contralateral and ipsilateral AAV-A53T injected mice treated with saline (A-D), or 2a (G-L) magnification 120X and scale bar is 40 mm; M-S) double immunofluorescence of CI-MPR (green) in TH-positive DA neurons (red) in the ipsilateral SNpc of AAV-A53T injected mice treated with saline (M-O) or 2a (P-R) at higher magnification 240X and scale bar is 10 mm; S) Graph indicates the fluorescence intensity (arbitrary units) of CI-MPR in DA neurons in SNpc, each dot represents an individual DA neuron in the SNpc (n=6–8 mice per group); T) Western blot of Sortilin protein levels in contralateral and ipsilateral SN brain extracts from AAV-A53T injected mice; U) relative Sortilin protein levels normalized to b-Actin (8 mice per group) in the ipsilateral and contralateral SN of AAV-A53T injected mice treated with saline (white) or 2a (grey) (n=4 mice per group); Significance determined by one-way ANOVA. Error bars represent mean ± SD.