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[Preprint]. 2023 Oct 9:rs.3.rs-3405677. [Version 1] doi: 10.21203/rs.3.rs-3405677/v1

Figure 4. UMCD6 alters gene expression in human NK cells to enhance cytotoxic function.

Figure 4

A: RNA-seq data obtained using NK-92 cells stimulated with UMCD6 in a 6-hour culture shows widespread changes in gene expression of 180 genes. B: Up-regulation of the activating NK receptors NKG2D (Klrk1), DAP10 (Hcst) and 2B4 (CD244), shown to be involved in the activation of NK cells, was confirmed by RT-PCR. C: Schematic representation of the role of UMCD6 in the activation of NK cells. Internalization of CD6 by UMCD6 up-regulates the expression of the activating receptor NKG2D-DAP10 and PI3K pathway. D: PI3K and mTOR expression, down-stream pathways of NKG2D-DAP10 signaling complex, were found up-regulated at protein level in a 72-hour co-culture of NK-92 cells with UMCD6. Data expressed as mean +/− SD and *p<0.05.