Table 1:
Results from two-way ANOVAs demonstrating the main effect of genotype, main effect of isolation protocol, and interaction on the raw counts and relative frequencies of each immune cell population. These statistics accompany the experiment presented in Figure 1.
| Genotype effect | Protocol effect | Interaction | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| *F*[1,6] | *p* | *F*[1,6] | *p* | *F*[1,6] | *p* | |
|
| ||||||
| Live cells | ||||||
|
| ||||||
| Raw count | 3.387 | 0.115 | 36.232 | 0.001 | 9.039 | 0.024 |
|
| ||||||
| % of live cells | 11.064 | 0.016 | 0.161 | 0.702 | 0.234 | 0.646 |
|
| ||||||
| CD45− | ||||||
|
| ||||||
| Raw count | 0.781 | 0.411 | 0.223 | 0.653 | 0.198 | 0.672 |
|
| ||||||
| % of live cells | 0.547 | 0.487 | 962.306 | < 0.001 | 33.516 | 0.001 |
|
| ||||||
| Microglia | ||||||
|
| ||||||
| Raw count | 9.930 | 0.02 | 81.385 | < 0.001 | 12.589 | 0.012 |
|
| ||||||
| % of live cells | 6.052 | 0.049 | 118.337 | < 0.001 | 0.892 | 0.381 |
|
| ||||||
| Dendritic cells | ||||||
|
| ||||||
| Raw count | 1.690 | 0.241 | 14.219 | 0.009 | 1.769 | 0.232 |
|
| ||||||
| % of live cells | 0.070 | 0.801 | 18.916 | 0.005 | 0.076 | 0.791 |
|
| ||||||
| Monocytes/macrophages | ||||||
|
| ||||||
| Raw count | 3.434 | 0.113 | 32.274 | 0.001 | 6.139 | 0.048 |
|
| ||||||
| % of live cells | 0.009 | 0.927 | 126.680 | < 0.001 | 3.163 | 0.126 |
|
| ||||||
| T-cells | ||||||
|
| ||||||
| Raw count | 7.243 | 0.036 | 0.440 | 0.532 | 0.756 | 0.418 |
|
| ||||||
| % of live cells | 5.199 | 0.063 | 1.761 | 0.233 | 1.692 | 0.241 |
|
| ||||||
| B-cells | ||||||
|
| ||||||
| Raw count | 0.734 | 0.425 | 4.441 | 0.08 | 0.868 | 0.388 |
|
| ||||||
| % of live cells | 0.068 | 0.802 | 3.544 | 0.109 | 0.208 | 0.664 |
|
| ||||||
| Neutrophils | ||||||
|
| ||||||
| Raw count | 0.242 | 0.641 | 0.768 | 0.414 | 0.136 | 0.725 |
|
| ||||||
| % of live cells | 1.903 | 0.217 | 1.902 | 0.217 | 0.779 | 0.411 |