Figure 1.

GPR55 RNAscope ISH in WT and GPR55-KO mice. A/B: GPR55 mRNA was detected in the VTA in WT mice (A), but not in GPR55-KO mice (B). However, GPR55 did not show colocalization with TH mRNA, a DA neuronal marker, in the VTA of WT mice (A). C/D: High density GPR55 mRNA was also detected in the PFC of WT mice (C), but not in GPR55-KO mice (D). Notably, GPR55 mRNA colocalizes with VgluT1 mRNA, a cortical glutamatergic neuronal marker, in the PFC of WT mice (A). (Also see Figs. S1-S2)