Figure 3.

Cold‐induced pigment improves the growth of dense patches of E. coli under illumination at 32 °C. a) White light transillumination image of patches of E. coli containing either our temperature switch construct or an unpigmented control construct encoding heat‐inducible GFP after transferring to agar for imaging at 48 h. b) Thermal IR image of patches inside illuminated growth chamber at 48 h. c,d) Representative OCT images of patches of E. coli containing our temperature c) switch construct, or d) the unpigmented control construct. e,f) Thickness of patches grown e) under illumination, or f) in a dark incubator over time. The slower rate of evaporation without illumination allows for thicker growth overall than with illumination. g,h) Area of patches grown g) under illumination or h) in a dark incubator over time. n = 4 biological replicates; error bars represent +/− standard error of the mean.