Figure 7.

ALI promotes physiologically‐relevant gene expression patterns in EnAos. a) UMAP analysis of integrated scRNA‐Seq data from ALI‐EnAos and SC‐EnAos (both under E₂ and E₂+P4+cAMP treatment with ALI 4D). b) Volcano plot of differentially expressed genes (DEGs) in EnSCs between ALI‐EnAos and SC‐EnAos. c) UMAP plots of representative decidualization genes in EnSCs of ALI‐EnAos and SC‐EnAos (The percentage represents the proportion of positively expressed cells). d) Violin plots of ciliogenesis‐related genes in ciliated cells of ALI‐EnAos and SC‐EnAos. e) Heat maps of DEGs in unciliated cells of ALI‐EnAos and SC‐EnAos under E₂ and E₂+P4+cAMP treatment to minic different phases. Some representative genes were shown. Gene expression levels were normalized. f) Differential expressions of some representative window of implantation (WOI) genes in ALI‐EnAos and SC‐EnAos under E₂ (D4) and E₂+P4+cAMP (D4) treatment to minic Pro‐phase and Sec‐phase. Gene expression levels were normalized. g) Related genes of WNT signaling pathway in epithelium of ALI‐EnAos and SC‐EnAos. h) Schematic diagram of the ALI‐cultured EnAos mimicking proliferative and secretory endometrium.