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. 2023 Jul 11;193(3):1758–1771. doi: 10.1093/plphys/kiad402

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© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Substrate specificity and kinetic parameters of AgApiT. A) Donor substrate specificity of AgApiT. The purified recombinant AgApiT was reacted with 50 μ M apigenin 7-O-glucoside and 1 mM of each sugar nucleotide. B) Acceptor substrate specificity of AgApiT. The purified recombinant AgApiT was reacted with 50 μ M of each acceptor substrate and 1 mM UDP-Api. The activities are presented as mean values with Se of 3 independent samples. Michaelis–Menten plots of AgApiT for C) UDP-Api and D) apigenin 7-O-glucoside. The mean values with Sd (of 3 independent samples) of the velocity at each substrate concentration were plotted. The K_m and k_cat values were calculated from the nonlinear regression curve shown using the solid line.