Figure 3.

The deap2 mutant contains similarly reduced PSII levels as pam68.A) Immunoblot analysis of thylakoid protein corresponding to the same amount of chlorophyll (Chl) from wild type (WT), cDEAP2-1, deap2-1, and pam68-2 using specific antibodies against DEAP2 (1 µg Chl) and PAM68 (5 µg Chl), several subunits of photosystem II (PSII: D1, CP47, CP43, D2, PsbE, PsbH, PsbO, PsbR, PsbTc, all 1 µg Chl and PsbI, 5 µg Chl) and representative subunits of light-harvesting complex II (LHCII: Lhcb1, 1 µg Chl), photosystem I (PSI: PsaA, 1 µg Chl), cytochrome b₆f complex (Cyt: PetA, 1 µg Chl), ATP synthase (AtpB, 1 µg Chl), and NADPH-dehydrogenase complex (NDH: NdhB, 2 µg Chl). Ponceau red (P.R.) served as a control for equal loading. Numbers left of the immunoblots indicate molecular weight in kDa of protein standards. B) PSII, PSI, and Cyt b₆f were quantified spectroscopically in isolated thylakoids relative to total chlorophyll. Averages are shown for n = 5 to 7 ± SD. The capital letters above the graphs denote the two significantly different groups (P < 0.05) as determined by one-way ANOVA and Holm-Sidak multiple comparison test.