Table 3.
RP3VKISS1 neuron excitatory responses to 5-HT receptor agonists in female mice.
| TCB-2 | WAY161503 | SR57227 | Cisapride | WAY208466 | AS19 | |
|---|---|---|---|---|---|---|
| Total cell number | 37 | 68 | 84 | 78 | 75 | 54 |
| Excited cell number (proportion) | 20 (54.1%) | 48 (70.6%) | 16 (19.0%) | 26 (33.3%) | 14 (18.7%) | 12 (22.2%) |
| Fisher’s exact tests | p < 0.001 versus SR57227 and WAY208466 | p < 0.001 versus SR57227, cisapride, WAY208466 and AS19 | p < 0.05 versus cisapride | p < 0.05 versus TCB-2 | p < 0.05 versus cisapride | p < 0.01 versus TCB-2 |
| Peak Δnormalized fluorescence (excited cells only) | 1.33 ± 0.28% | 3.21 ± 0.28% | 0.54 ± 0.14% | 1.03 ± 0.29% | 0.37 ± 0.20% | 0.53 ± 0.14% |
| Kruskal-Wallis test a | p < 0.001, K-S statistic = 62.09 | |||||
| Dunn’s post-tests | / | p < 0.01 versus TCB-2 | p < 0.001 versus WAY161503 | p < 0.001 versus WAY161503 | p < 0.001 versus WAY161503 | p < 0.001 versus WAY161503 |
| Slice (mouse) number |
5 (4) | 5(3) | 7 (4) b | 6(3) | 5(3) b | 5(5) b |
a
the Kruskal-Wallis test was used to test for statistical differences across the effect of all 6 agonists (TCB-2, WAY161503, SR57227, cisapride, WAY208466 and AS19).
b
RP3VKISS1 cells showing excitatory responses to SR57227, WAY208466 and to AS19 were found in 6 slices from 4 mice, in 4 slices from 3 mice and in 3 slices from 3 mice, respectively.