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. 2023 Oct 16;24(11):1947–1959. doi: 10.1038/s41590-023-01641-8

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — (a) Surface protein marker profiles of naive and MNP-2 CD8 T cells. (b) Naive CD8 T cell activation experiment with TCR stimulation (anti-CD3/anti-CD28 beads (0.5 beads per cell)). Cells were assessed over a 7-day time course for MNP-2 surface markers (CD8-beta, CD244, CD11b, IL21R) and activation markers (CD69, CD25, CD71) by flow cytometry. (c) Representative plot of MNP-2 cells delineated by CD8-beta^Low and CD244^high co-expression pre- and post-isolation, as well as over a 7-day time course post-TCR stimulation. (d) Frequencies of CD8-beta^LowCD244^high MNP-2 cells over the TCR stimulation time course (n = 4–7 donors). Results are from three independent experiments. P-values were determined by one-way ANOVA with Holm-Sidak’s multiple comparisons test. ****P < 0.0001. Tukey’s box plots with median with 1^st and 3^rd quartiles shown. (e) Representative histograms of activation markers and (f) frequencies of positive cells for each activation marker over the TCR stimulation time course (n = 4 donors). Mean +/− sem.

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