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. 2023 Aug 31;51(19):10292–10308. doi: 10.1093/nar/gkad719

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — BEND3 represses Dazl expression. (A) Procedure for validation of Bend3 as a bona fide hit. (B) FACS analysis on serum-grown cells. (C) RT-qPCR on serum-grown cells. (D) Western blotting on serum-grown cells. (E) FACS analysis on serum-grown Bend3 mutant cells, rescued with empty vector or with a Bend3-expressing vector. (F) Survival assay on rescued cells. Only one of the three clones is shown, for clarity. (G) Western blotting shows expression of the V5-tagged BEND3 protein in rescue cells, and concomitant repression of DAZL protein expression. The three independent clones are shown.