Fig. 7.

The effects of GPR176 expression on the phenotypes and molecular mechanisms of oesophageal cancer cells. After transfection of shGPR176, GPR176 expression became weaker in KYSE-150 cells, as determined by western blotting (A). The transfectants were subjected to functional assays of proliferation, chemoresistance, apoptosis, migration and invasion using CCK-8 (B, C), Annexin V/7-AAD staining (D), wound healing assay (E) and transwell assay (F), respectively. The proteins associated with these phenotypes were screened by western blotting (G). KYSE, KYSE-150 cells; *p < 0.05; **p < 0.01; ***p < 0.001