Fig. 1.

Algorithm for Multimodal Spectroscopic Signature acquisition from cardiac patient samples. Fresh tissue was acquired from 15 patients at time of cardiac transplantation, either from explanted dilated and ischaemic cardiomyopathy patients or healthy hearts at time of organ retrieval. Samples were scanned using separate Raman (3 scans per sample) and NIRS (4 scans per sample) instruments to acquire an MSS (combines the 7 scans per sample, 35 in total for each pathology), and then entered into machine learning (ML) algorithms to assess their diagnostic accuracy. H&E haematoxylin and eosin, PSR picrosirius red, VG Van Gieson. Created with BioRender.com