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. 2023 Oct 26;8:163. doi: 10.1038/s41541-023-00759-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Sera from C57BL/6J mice immunized on days 0 and day 21 with excipient, antigen (Ag) alone or formulations containing antigen and various adjuvants were collected on day 42. Surrogate virus neutralizing titers were quantified by measuring inhibition of RBD binding to human ACE2 receptor. (Box-whisker plots: centre line: median; bounds: 25th/75th percentile; whiskers; lowest/highest point) Significance was calculated using a two-way repeated measures ANOVA with Tukey’s multiple comparison testing. N = 4–6 (*p < 0.05, **p < 0.01, ***p < 0.001), ****p < 0.0001).