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. 2023 Oct 26;14:6827. doi: 10.1038/s41467-023-42252-z

Fig. 2. taRNAs enhance endogenous gene expression.

Fig. 2

Two gRNAs were tested in PTV-based taRNAs for each endogenous mRNA target, including a human PTEN; b human PPIB; c human CDKN1A; d human ABCA7. Each gRNA is annotated with where their last nucleotide binds relative to the stop codon on the target transcript. All experiments were done in HEK293T cells transfected with plasmids expressing the indicated taRNAs and harvested for western blots after 48 h. GAPDH or α-tubulin was used as the loading control. Representative blots were shown as top panels, and quantifications normalized to non-targeting control (NT) were shown below. All bar-graph values are shown as mean ± SEM with data points. n = 4 biological replicates for (a) and (c). n = 3 biological replicates for (b) and (d). Statistical analyses were performed using one-way ANOVA with Dunnett’s multiple comparison test vs. NT. *P < 0.05, **P < 0.01. No asterisk = not significant. The P value and source data are provided as a Source Data file.