Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 26;13:18341. doi: 10.1038/s41598-023-44050-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Enrichment and characterization of cell line and tissue explant EVs. (a) Surgical resections of healthy FT or tumor tissues were minced and used to initiate short-term tissue explants (cultured for 24 h) followed by collection of the conditioned media and processed by differential ultracentrifugation to enrich for EVs. Likewise, conditioned media from the FT and ovarian cancer cell lines shown was collected and processed. Created with BioRender.com (b) Representative NTA data. (c) Sixty (60) EV particles were imaged, and their size was measured for representative samples by TEM at x30K magnification. The mean size with s.e.m. is indicated by the error bars. (d) Shown are representative fluorescence data obtained using ExoView for FT and HGSOC tissue explant derived EVs. The EVs were captured using commonly expressed EV tetraspanins, namely, CD9, CD63 and CD81 and probed with detection antibodies conjugated to Alexa Fluor dyes: CD9-AF488 (blue), CD63-AF647 (pink) and CD81-AF555 (green). The error bars represent the mean particle count with s.e.m.