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. 2023 Oct 26;13:18341. doi: 10.1038/s41598-023-44050-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of predicted transmembrane proteins in FT and HGSOC cell line EVs using capillary western blotting. One antibody for each of the 45 candidate transmembrane proteins was evaluated by capillary western blotting. The 7 exo-proteins that were confirmed to be present in EVs from 6 HGSOC and 3 FT cell lines are shown. Excluded from the figure are 23 exo-proteins with non-specific bands or no detectable bands at the expected molecular weight, and 15 exo-proteins with bands at the expected molecular weight but did not meet the criteria for presence in the EVs of all tested cell lines. In addition, CD81 and FLOT1 were evaluated as these are common EV markers. Full length unprocessed blots can be found in Supplementary Fig. 6.