Fig. 1.
Conditional deletion of Wwtr1 in mature osteoblasts on postnatal bone homeostasis. a, b Bone mineral density by DEXA scan in male and female mice. c Bone structure by micro-CT 3D images analysis from both male and female mice. d Periosteal mineral apposition rate (MAR) by calcein double labeling. There was a significant reduction in periosteal MAR in single Wwtr1Oc-Het heterozygous mice compared with age-matched control mice and an even greater decrement in Wwtr1Oc-cKO null mice, indicating a gene-dosage effect of Wwtr1 on osteoblast-mediated bone formation. e TRAP staining (red color) for osteoclast activity. Data are expressed as the mean ± S.D. from serum samples of individual mice (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 compared with wild-type control mice. P values were determined by 1-way ANOVA with Tukey’s multiple-comparisons test