Fig. 2.

Conditional deletion of Pkd1 and Wwtr1 in mature osteoblasts on postnatal bone homeostasis. a, b Bone mineral density by DEXA scan in male and female mice. c Bone structure by micro-CT 3D images analysis from male mice. d Periosteal mineral apposition rate (MAR) by Calcein double labeling. There was a significant reduction in periosteal MAR in single Pkd1^Oc-cKO or TAZ^Oc-cKO mice compared with age-matched control mice and an even greater decrement in double Pkd1/TAZ^Oc-cKO null mice, indicating an additive effect of PC1 and TAZ on osteoblast-mediated bone formation. e TRAP staining (red color) for osteoclast activity. Data are expressed as the mean ± S.D. from serum samples of individual mice (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 compared with wild-type mice, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared with Wwtr1^Oc-cKO mice, and ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 compared with Pkd1^Oc-cKO mice, respectively. P values were determined by 1-way ANOVA with Tukey’s multiple-comparisons test