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. 2023 Oct 26;11:57. doi: 10.1038/s41413-023-00295-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cell-based BRET² assays for MS2-target engagement assays. a Chemical structure of MS2 and an example of predicted 3D binding mode for MS2 (ball-and-sticks rendering in CPK colors) in PC2 (light blue). PC1 (red) as bound to PC2 in the homology structure is superimposed. b An example of calculated 2D binding mode and residues for MS2 in PC1/PC2 C-tails. c A diagram of BRET² constructs and reactions in the presence of DeepBlue C with or without MS2 stimulation. d BRET² signal changes from wild-type and mutant constructs with or without MS2 incubation. e, f Time-dependent changes of PC1 and PC2 proteins with or without MS2 incubation during osteogenic differentiation cultures in MC3T3-E1 cell line. Incubation Data are presented as the mean ± SD from 3 independent experiments (n = 3). **P < 0.01 compared with vehicle control