Table 1.

Gene-expression profiles in bone from Wwtr1-deficient mice

Gene	Wild-type	Wwtr1+/−	Wwtr1Oc-Het	Wwtr1Oc-cKO	P-value
Osteoblast lineage
Pkd1	1.00 ± 0.15	1.03 ± 0.22	0.99 ± 0.34	0.96 ± 0.32	0.971 8
Wwtr1	1.00 ± 0.24	0.61 ± 0.10***	0.63 ± 0.14***	0.36 ± 0.09***	<0.000 1
Fgf23	1.00 ± 0.10	0.97 ± 0.22	1.61 ± 0.45*	2.31 ± 0.61***	<0.000 1
Runx2-II	1.00 ± 0.13	0.80 ± 0.21	0.82 ± 0.27	0.61 ± 0.10**	0.017 7
Osteopontin	1.00 ± 0.26	0.83 ± 0.20	0.67 ± 0.11*	0.62 ± 0.21*	0.014 3
Bsp	1.00 ± 0.11	0.94 ± 0.28	0.68 ± 0.16*	0.54 ± 0.15***	0.000 3
Mepe	1.00 ± 0.12	1.04 ± 0.22	1.43 ± 0.28**	1.81 ± 0.22***	<0.000 1
Col1	1.00 ± 0.20	0.81 ± 0.23	0.67 ± 0.21*	0.65 ± 0.18*	0.030 4
Alp	1.00 ± 0.20	1.01 ± 0.29	0.69 ± 0.11*	0.52 ± 0.15***	0.000 2
Osteocalcin	1.00 ± 0.19	0.80 ± 0.23	0.53 ± 0.19**	0.57 ± 0.15**	0.001 3
OPG	1.00 ± 0.13	0.97 ± 0.29	0.89 ± 0.31	0.96 ± 0.24	0.892 6
RankL	1.00 ± 0.17	1.13 ± 0.24	1.12 ± 0.11	1.52 ± 0.15***	<0.000 1
Fzd2	1.00 ± 0.25	1.02 ± 0.18	1.03 ± 0.18	0.96 ± 0.28	0.935 4
Wnt10b	1.00 ± 0.08	1.08 ± 0.28	0.97 ± 0.17	0.73 ± 0.06*	0.016 4
CYR61	1.00 ± 0.13	1.06 ± 0.10	1.54 ± 0.25**	2.06 ± 0.55***	<0.000 1
CTGF	1.00 ± 0.10	1.06 ± 0.28	1.18 ± 0.41	1.54 ± 0.31*	0.022 1
Osteoclast
Trap	1.00 ± 0.15	0.95 ± 0.19	1.10 ± 0.22	1.41 ± 0.19**	0.002 1
Mmp9	1.00 ± 0.14	0.85 ± 0.22	0.87 ± 0.15	0.86 ± 0.18	0.380 8
Chondrocyte
Collagen II	1.00 ± 0.20	0.86 ± 0.20	0.79 ± 0.26	0.67 ± 0.15*	0.045 8
Collagen X	1.00 ± 0.21	0.87 ± 0.36	0.74 ± 0.39	0.45 ± 0.23*	0.033 9
Adipocyte
PPARγ2	1.00 ± 0.17	1.23 ± 0.14	1.57 ± 0.30**	2.03 ± 0.42***	<0.000 1
aP2	1.00 ± 0.18	1.27 ± 0.31	1.64 ± 0.29**	2.51 ± 0.36***	<0.000 1
Lpl	1.00 ± 0.15	1.31 ± 0.37	1.63 ± 0.34*	2.56 ± 0.47***	<0.000 1

Data are mean ± S.D. from 6 tibias of wild-type control, Wwtr1^+/−, Wwtr1^Oc-Het, and Wwtr1^Oc-cKO mice and expressed as the fold changes relative to the housekeeping gene β-actin subsequently normalized to control mice. *, **, *** indicates significant difference from wild-type control at P < 0.05, P < 0.01, P < 0.001, respectively. P values were determined by 1-way ANOVA with Tukey’s multiple-comparisons test