Table 2.

Gene-expression profiles in bone from Pkd1^Oc-cKO or/and Wwtr1^Oc-cKO mice

Gene	Wild-type	Pkd1Oc-cKO	Wwtr1Oc-cKO	Pkd1/Wwtr1Oc-cKO	P-value
Osteoblast lineage
Pkd1	1.00 ± 0.11	0.41 ± 0.10***	0.93 ± 0.31	0.45 ± 0.10&&&	<0.000 1
Wwtr1	1.00 ± 0.24	0.98 ± 0.27	0.46 ± 0.10***	0.43 ± 0.15###	<0.000 1
Pkd2	1.00 ± 0.12	1.05 ± 0.21	0.93 ± 0.25	1.09 ± 0.31	0.627 1
Runx2-II	1.00 ± 0.14	0.76 ± 0.11**	0.75 ± 0.10*	0.62 ± 0.21#,&	0.001 7
Osteocalcin	1.00 ± 0.13	0.75 ± 0.15**	0.78 ± 0.11**	0.66 ± 0.11#,&	0.001 0
FGF-23	1.00 ± 0.13	0.55 ± 0.17*	2.30 ± 0.56***	1.02 ± 0.25	<0.000 1
Dmp1	1.00 ± 0.20	0.80 ± 0.10*	0.78 ± 0.14*	0.65 ± 0.10#,&	0.001 2
Wnt10b	1.00 ± 0.04	0.77 ± 0.20**	0.73 ± 0.19**	0.63 ± 0.11#,&	0.001 4
Wnt1	1.00 ± 0.21	0.70 ± 0.16*	0.92 ± 0.35	0.52 ± 0.21#,&&&	0.000 7
FzD2	1.00 ± 0.33	0.92 ± 0.17	0.93 ± 0.24	0.55 ± 0.19#,&	0.001 6
c-Jun	1.00 ± 0.24	0.95 ± 0.28	1.07 ± 0.22	0.62 ± 0.12#,&&	0.000 9
c-Fos	1.00 ± 0.20	0.92 ± 0.21	0.74 ± 0.15*	0.67 ± 0.13###	0.001 1
Axin-2	1.00 ± 0.31	0.77 ± 0.10*	0.88 ± 0.20	0.67 ± 0.13&	0.001 2
PTGS2	1.00 ± 0.27	0.70 ± 0.15*	0.68 ± 0.16**	0.44 ± 0.19#,&	<0.000 1
OPG	1.00 ± 0.19	0.93 ± 0.26	1.08 ± 0.26	0.97 ± 0.18	0.708 0
RankL	1.00 ± 0.15	0.73 ± 0.14*	1.45 ± 0.13***	0.89 ± 0.18#,&&&	<0.000 1
CYR61	1.00 ± 0.13	1.40 ± 0.18*	1.94 ± 0.51***	1.67 ± 0.33	0.000 5
CTGF	1.00 ± 0.10	1.38 ± 0.23*	1.68 ± 0.30**	1.55 ± 0.36	0.001 4
Osteoclast
Trap	1.00 ± 0.15	0.63 ± 0.14**	1.43 ± 0.20***	0.93 ± 0.14##,&&&	<0.000 1
Mmp9	1.00 ± 0.14	0.63 ± 0.08*	0.86 ± 0.18	0.95 ± 0.35#	0.031 7
Chondrocyte
Collagen II	1.00 ± 0.19	0.92 ± 0.24	0.73 ± 0.15*	0.51 ± 0.07##,&	<0.000 1
VegfA	1.00 ± 0.38	1.74 ± 0.22*	1.66 ± 0.49*	1.70 ± 0.57	0.014 3
Adipocyte
PPARγ2	1.00 ± 0.20	1.78 ± 0.34**	1.88 ± 0.51**	1.61 ± 0.22	0.001 0
aP2	1.00 ± 0.16	1.71 ± 0.33***	2.09 ± 0.31***	1.77 ± 0.38	<0.000 1
Lpl	1.00 ± 0.13	1.62 ± 0.28**	2.01 ± 0.51***	1.88 ± 0.38	0.000 4

Data are mean ± S.D. from 6 tibias of wild-type control, Pkd1^Oc-cKO, Wwtr1^Oc-cKO, and Pkd1/Wwtr1^Oc-cKO mice and expressed as the fold changes relative to the housekeeping gene β-actin subsequently normalized to control mice. *P < 0.05, **P < 0.01, ***P < 0.001 compared with wild-type mice, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared with Wwtr1^Oc-cKO mice, and ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 compared with Pkd1^Oc-cKO mice, respectively. P values were determined by 1-way ANOVA with Tukey’s multiple-comparisons test