Table 1.
The sequence of primers used in this study.
| Gene | Sequence 5′-3′ | Annealing Tem. (°C) | References |
|---|---|---|---|
| CD44 (F) | AAGGTGGAGCAAACACAACC | 60 | 32 |
| CD44 (R) | AGCTTTTTCTTCTGCCCACA | 60 | |
| ANXA1 (F) | GATTCAGATGCCAGGGCCT | 60 | 33 |
| ANXA1 (R) | CACTCTGCGAAGTTGTGGAT | 60 | |
| KTR18 (F) | GTGGTGCTCTCCTCAATC | 60 | 33 |
| KTR18 (R) | GCTCTGGGTTGACCGTGG | 60 | |
| SOX2 (F) | CCCAGCAGACTTCACATGT | 60 | 33 |
| SOX2 (R) | CCTCCCATTTCCCTCGTTTT | 60 | |
| OCT4 (F) | CCTCACTTCACTGCACTGTA | 60 | 34 |
| OCT4 (R) | CAGGTTTTTCTTTCCCTAGCT | 60 | |
| GAPDH (F) | TCTCCTCTGACTTCAACAGCGAC | 60 | 35 |
| GAPDH (R) | CCCTGTTGCTGTAGCCAAATTC | 60 | |
| miR-144-5p (RT) | GTTGGCTCTGGTGCAGGGTCCGAGG | 60 | Designed by Authors |
| TATTCGCACCAGAGCCAACCTTACA | |||
| miR-144-5p (F) | GTTGGGGGATATCATCATATAC | 55 | Designed by Authors |
| miR-146a-5p (RT) | GTTGGCTCTGGTGCAGGGTCCGAGG | 42 | |
| TATTCGCACCAGAGCCAACAACCCA | |||
| miR-146a-5p (F) | GTTTGGTGAGAACTGAATTCCA | 55 | Designed by Authors |
| miR-155-5p (RT) | GTTGGCTCTGGTGCAGGGTCCGAGG | 42 | |
| TATTCGCACCAGAGCCAACACCCCT | |||
| miR-155-5p (F) | GTGGGTTAATGCTAATCGTGAT | 55 | Designed by Authors |
| miR-21-5p (RT) | GTTGGCTCTGGTGCAGGGTCCGAGG | 42 | |
| TATTCGCACCAGAGCCAACTCAACA | |||
| miR-21-5p | GTTTGGTAGCTTATCAGACTGA | 55 | Designed by Authors |
| U6 snRNA (RT) | GTTGGCTCTGGTGCAGGGTCCGAGG | 55 | |
| TATTCGCACCAGAGCCAACAAAATA | |||
| U6 snRNA (F) | TTGGTGCTCGCTTCGGCA | ||
| Universe miRNA | GTGCAGGGTCCGAGGT |
R: Reverse; F: Forward; RT: Reverse Transcriptase; Temp: Temperature.