Table 1.
Cell surface markers of interest used in the broad immune full spectrum flow for the Immunology of THymectomy And Childhood CArdiac transplant study
| Cell surface marker | Fluorophore | Antibody clone | Purpose |
| CD45 | Spark YG 593 | HI03 | Leucocytes |
| TCRγd | PerCP-eFluor 710 | B1.1 | γd T-lymphocyte |
| CD3 | BV 510 | SK7 | Pan T-cell, NK T-like cells |
| CD4 | PerCP | SK3 | CD4 T-helper lymphocytes |
| CD8 | BUV 805 | SK1 | CD8 cytotoxic T-lymphocytes |
| CD11c | PE-Cy7 | B-ly6 | Dendritic cell differentiation |
| CD14 | Spark Blue 550 | 63D3 | Monocyte differentiation |
| CD16 | BUV 496 | 3G8 | Monocyte, NK cell and dendritic cell differentiation |
| CD19 | Spark NIR 685 | HIB19 | B lymphocytes |
| CD20 | BV 786 | 2H7 | B lymphocytes |
| CD21 | PE-Cy5 | B-ly4 | B lymphocyte differentiation |
| CD24 | PE-AF 610 | SN3 | B lymphocyte differentiation |
| CD27 | APC-H7 | M-T271 | T/B lymphocyte differentiation |
| CD38 | APC-Fire 810 | HIT2 | Monocyte, dendritic cell, T/B lymphocyte activation and differentiation |
| CD56 | BUV 737 | NCAM16.2 | NK cells |
| CD57 | Pacific Blue | NK-1 | NK and CD8 T lymphocyte immune senescence |
| CD123 | Super Bright 436 | 6H6 | Plasmacytoid dendritic cells |
| CD127 | APC-R700 | HIL-7R-M21 | Cytokine receptor; T lymphocyte differentiation |
| KIR (CD158) | BUV 605 | DX27 | NK cells |
| NKG2A (CD159a) | APC | REA110 | NK cells |
| NKG2C (CD159c) | PE | REA205 | NK cells |
| IgD | BV 480 | IA6-2 | B lymphocyte differentiation |
| IgM | BV 570 | MHM-88 | B lymphocyte differentiation |
| HLA-DR | PE-Fire 810 | L243 | Monocyte activation, dendritic cell lineage, NK cell lineage discrimination, |
| Viability | Live/Dead Blue | – | Live cells |
NK, Natural Killer.