Table 2.
Cell surface markers of interest used in the T lymphocyte full spectrum flow panel for the Immunology of THymectomy And Childhood CArdiac transplant study
| Cell surface marker | Fluorophore | Antibody clone | Purpose |
| CD45 | Spark YG 593 | HI03 | Leucocytes |
| CD45RA | BUV 395 | 5H9 | T lymphocyte differentiation |
| CD3 | BV510 | SK7 | Pan T-cell, NK T-like cells |
| CD4 | PerCP | SK3 | CD4 T-helper lymphocytes |
| CD8 | BUV 805 | SK1 | CD8 cytotoxic T lymphocytes |
| TCRγd | PerCP-eFluor 710 | B1.1 | γd T lymphocyte |
| CD25 | PE-AF700 | CD25-3G10 | Regulatory T lymphocytes |
| CD27 | APC-H7 | M-T271 | T lymphocyte differentiation |
| CD28 | BV650 | CD28.2 | T lymphocyte differentiation |
| CD31 | BV711 | WM59 | Recent thymic emigrants |
| CD38 | APC-Fire 810 | HIT2 | T lymphocyte activation/differentiation |
| CD39 | BUV 661 | TU66 | Regulatory T lymphocytes |
| CD57 | Pacific Blue | NK-1 | CD8 T lymphocyte immune senescence |
| CD62L | BUV 496 | SK-11 | T lymphocyte differentiation |
| CD69 | AF 647 | FN50 | T lymphocyte activation |
| CD95 | PE-Cy5 | DX2 | T lymphocyte activation/differentiation |
| CD127 | APC-R700 | HIL-7R-M21 | Chemokine receptor; T lymphocyte differentiation |
| HLA-DR | PE-Fire 810 | L243 | T lymphocyte activation |
| CCR4 | BB 700 | 1G1 | Chemokine receptor; T lymphocyte differentiation |
| CCR5 | BUV 563 | 2D7/CCR5 | Chemokine receptor; T lymphocyte differentiation |
| CCR6 | BV 480 | 140 706 | Chemokine receptor; T lymphocyte differentiation |
| CCR7 | BV 421 | G043H7 | T lymphocyte differentiation |
| CXCR3 | AF 488 | GH025H7 | Chemokine receptor; T lymphocyte differentiation |
| CXCR5 | BV 750 | RF8B2 | Chemokine receptor; T lymphocyte differentiation |
| PD-1 | BV 785 | EH12.2H7 | Co-inhibitory receptor; T lymphocyte exhaustion |
| LAG-3 | PE-Cy7 | 3DS223H | Co-inhibitory receptor; T lymphocyte exhaustion |
| TIM-3 | BV 605 | F38-2E2 | Co-inhibitory receptor; T lymphocyte exhaustion |
| CTLA4 | PE-CF594 | BNI3 | Co-inhibitory receptor; T lymphocyte exhaustion |
| Tetramer (lytic) | APC | – | EBV-specific T lymphocytes |
| Tetramer (latent) | PE | – | EBV-specific T lymphocytes |
| Viability | Live/Dead Blue | – | Live cells |
EBV, Epstein-Barr virus; NK, Natural Killer.