Table 2.
Primers used for the qRT-PCR assays. CYP: cytochrome P450; SOD: superoxide dismutase; CAT: catalase; HSP70: heat shock protein 70. F: forward primer; R: reverse primer. BP: base pairs. The annealing temperature of all reactions was 60 °C.
| Gene | Initial | Sequence | Genbank Access Entries |
|---|---|---|---|
| Cytochrome P450 | CYP | F: 5′-GCCAAGGGACACGATTCTAC-3′ R: 5′-CATACAAAGACACCGCCATTC-3′ |
OP066521 |
| Superoxide dismutase | SOD | F: 5′-TGATTCGTAGGGACTTTGGA-3′ R: 5′-CCTGGTTAGCACAAGCAACA-3′ |
OP066522 |
| Catalase | CAT | F:5′-CTCTCAAGTCGGTGTATTCTGG-3′ F:5′-CCTGTTTTTCCTTTCGCTGT-3′ |
OP066523 |
| Heat Shock Protein 70 | HSP70 | F:5′-GAAGCCTACCTCGGACAAAA-3′ | OP066524 |
| F:5′-CGGACCTCAAACAGTGAACC-3′ | |||
| Glyceraldehyde 3-phosphate dehydrogenase | GAPDH * | F: 5′-TACTGCGAGGCAACAATAGG-3′ | OP066520 |
| R: 5′-GTTTTTCAAGGCACCACAGA-3′ |
* The primer GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) was the reference.