Table 2.

qPCR protocol for gene expression analysis.

Step	Time [s]	Temperature [°C]	Cycle
Preincubation	300	95	1
Denaturation	10	95
Annealing	15	Ta *	40
Elongation	20	72
Detection	-	72
Melting curve	5	95
	60	65	1
Cooling	60	40	1

* T_a: primer-specific annealing temperature.